- Curriculum Vitae
Roberto Corradini is Full Professo of Organic Chemistry since 2018 at the University of Parma, and is Coordinator of the Doctorate in Chemical Sciences since 2017. from 2000 to 2018 he has been Associate Professor of Organic Chemistry at the University of Parma. He has received the 2013-Italian National Scientific Qualification (ASN) for Full Professorship in Organic Chemistry.
Education. 1987: Laurea cum Laude at the University of Parma. 1988-1991: PhD in Chemical Sciences at the University of Parma.
Career. 1991: Research activity at the Laboratoire de Biophysique of the Museum National d'Histoire Naturelle, under the supervision of Claude Hélène. 1993-1994: Post Doctoral fellow at the University of Parma.1995-2000: Researcher, University of Parma.
Bibliometric data (march 2018) N. of papers on International ISI Journals (all databases) Total: 146; N. citations (ISI): 3571 N. citations without self-citations (ISI) : 2961. Hi (WOS): 33; Hi (Scopus): 35; book chapterd: 18.
N. international patents: 1 N National Patents: 1 (application december 2017). RC’s publications are presently above the thresholds for Associate professors, Full Professor, and Evaluator (Commissario) established for the ASN nantional evaluation.
Awards and Honours: 2015: RESEARCH - AWARD for “Organic Chemistry in its aspects of structural determination and molecular interactions” of the Organic Chemistry Division of the Italian Chemical Society. 1992:Chirality Prize by Waters Italy.
Research projects: 2017- Local coordinator for the MSCA-RISE Oligo Nanomed project (PI: Luisa de Cola, University of Strasbourg); 2015-Local Responsible for activities of linked third party and WP leader in the H2020 Project ULTRAPLACAD (coordiator: Prof. Giuseppe Spoto); 2011-2014 Coordinator of a National Research Project (PRIN09); 2012-2014: local coordinator of a project-FILAS-CR-2011-1054 (Lazio Region). 2012-Local Coordinator of PNA-NOVA project (Spinner Consortium-Emilia Romagna); RC participated as scientist to EU (2), National (8) regional (2) projects.
Invited Lectures. International : 1) Chirality 2001- Nat. Japanese Symposium on Chirality, July 2001, Osaka, Japan; 2) 19-Symposium on Chirality ISCD-19 at San Diego (U.S.A) July 2007; 3) 6-10th Intern. Symposium on Immunological, Chemical and Clinical Problems of Food Allergy”, Parma May 2008; 4) VII Spanish Italian Symposium on Organic Chemistry (VII SISOC), Oviedo (Spagna) September 2008; 5) 19-Symposium on Chirality 2009-ISCD 21 (Breckenridge, Colorado, USA) July 2009; 6) World Congress on Drug Discovery and Therapy 2013 (Boston, June 2013). 7) COST Action TD1003 "Bio-inspired Nanotechnologies for Biosensing" meeting, 16/5/2013 (Sitges, Spain); 8) FEBS Workshop “Decoding non-coding RNA in development and cancer”, 12-16 october, 2014 (Capri, Italy); 9) Biophotonics 2015, 3rd Intern. Conference on Biophotonics, Firenze 20-22 May, 2015. National Congresses: 1) Biotech.Org “Chimica organica e Biotecnologie: Sfide e Opportunità” Forte dei Marmi (LU) Maggio 2009; 2) XXXVI Convegno Naz. della Divisione di Chimica Organica, Bologna, 13-17 Settembre 2015 (Award lecture) 3) SUPRAMOL 2017 13th Italian Conference on Supramolecular Chemistry, S. Margherita di Pula (Ca) 18-21/6/2017. He also has also held invited lectures to private companies (4), universities (7), national advanced schools/workshops(10).
Editorial Activity:1-2017- Guest Editor of a Special issue of the Journal Molecules (MDPI) on PNA chemistry and properties; 2-2010-2015: Member of the Editorial Board Chirality (Wiley-VCH). 3-2012-2016 member of the Editorial Board and Acquisition Editor of Artificial DNA, PNA & XNA (Landes then Taylor&Francis).
Congress Committee Membership. 1998: Organizing Committee of the NATO Advanced Research Workshop on Supramolecular Science- Lerici (SP), Italy. 2005: Organizing Committee (and Scientific Manager) of the 17 International Symposium on Chirality (ISCD-17), Parma. Organizing Committee of the IX National congress on Supramolecular Chemistry, Parma, September 2009. Scientific Committee of the Workshop “Biophotonics 2011”, Parma, 8-10 June 2011. Track Chair-World Congress on Drug Discovery and Therapy (Boston, 3-6 june 2013), International Advisory Board-World Congress on Drug Discovery and Therapy (Boston, june 2014). Scientific Committee 1st Workshop “I Chimici per le Biotecnologie”-Bologna, 23-2-2018.
Activity as referee for journals e.g.: Nature Commun., J.l Am. Chem. Soc., Sci. Reports, Small, Chem. Eur. J.l, ChemBiochem, J. Org. Chem., Eur. J. Org. Chem., Org. Biomol. Chem.
Project evaluator for Institutions: Petroleum Research Fund (Washington, DC); Czech Science Foundation (Czech Republic); State Secretariat for Education and research-(Swiss federal Republic); Austrian Science Fund (Austria); United Arab Emirates University (Arab Emirates), ANVUR (Italy), Italian Consortium of Biotechnology (CIB, Italy), European Commission (REA).
Supervisor of 6 PhD theses in Chemical Sciences at the University of Parma, 1 of which has been awarded with a national prize (CINMPIS, 2012). Supervisor of two ongoing PhD theses in Chemical Sciences. External referee for PhD Theses for the University of Pune (India, 2008), University of Gent (Belgio, 2016) and Twente University (Olanda, 2016), Institute ISIS-University of Strasbourg (France, 2017) (for the last two RC has participated to the Defense Jury).
2000-today: Supervisor of Laurea Thesis in Chemistry (27), Food Science and Technology (10), Molecular Biology (2), and Industrial Biotechnology (1) at the University of Parma .
External Supervisor of a thesis in Biochemistry P.J. Šafárik University of Košice (Slovakia, 2006). Co-supervisor of Master thesis at the University of Gent (Belgium, 2014); 2000-today: Supervisor of first degree thesis (Laurea Triennale) in Chemical Sciences and Technologies (16), Biotechnology (27) and Food Science and Technology (1), University of Parma.
International collaborations (last 5 years). Peter E. Nielsen University of Copenhagen (DK); Luisa de Cola, ISIS-University of Strasbourg (F); Wolfgang Knoll-Austrian Institute of Technology, Vienna (A). Vincenzo Verdolino, ETH, Campus Lugano (CH). Mark M. Green, New York University (USA), Makoto Komiyama University of Tsukuba (Japan), Nina Berova, Columbia University (New York, USA), Juriaan Huskens, University of Twente (NL), Annemieke Madder, Università di Ghent (Belgio), Stavros Pissadakis, Foundation FORTH, Heraklion,(GR).
RC’s scientific activity has been dedicated to the study of molecular recognition of biologically active species, and in particular of enantiomers of chiral substances. In this field, he has carried out the following research lines: 1) Recogniton of enantiomers by chromatographic/electrophoretic methods.2) Chemo- and enantioselective fluorescent sensors based on cyclodextrins . 3) Cyclodextrin-based systems for toxin recognition. 4) Synthesis and structural properties of chiral peptide nucleic acids (PNAs). 5) Use PNAs in therapeutic applications with anti-gene and anti-miR activity. 6) PNA for advanced diagnostic systems; 7) PNA conjugated with nanostructured materials for theranostic applications. The points 4-7 are the most relevant for the project and will be illustrated in more detail.
4) Modified PNA monomers containing modified backbones with different stereochemistry were synthesised. In a milestone work in collaboration with Michele Saviano (Bari unit) the structure of a PNA:DNA duplex has been determined for the first time, and this allowed to rationally desing new PNAs. Modified PNAs were used in collaboration M. Komiyama (University of Tohoku) for the strand-invasion of dsDNA, allowing to obtain artificial restriction systems (ARCUT) with improved efficiency. PNA bearing modified nucleobases, in particular uracil derivatives, have been also produced by the group and some of these showed improved properties in terms of DNA affinity and sequence selectivity. Modelling studies based on molecular dynamics have been used to understand the effect of modification. The study of PNA:PNA duplexes has also been performed for several applicationsas PNA computing and for self-assembly of nanocomponents.
5) Both PNA and modified PNA have been synthesized and tested as potential drugs for the regulation of gene expression by antisense or anti-gene mechanisms. The latter strategy (targeting of dsDNA and inhibition of transcription) has been used to block the expression of a gene (MYCN) by antigene PNA both in cellular systems and in model animals. Research in this field has been recently directed towards the modulation of gene expression by PNA targeted to microRNA (miR). Modified PNA with chiral residues were shown to be particularly efficient in this application. Studies carried out in collaboration with Roberto Gambari’s group have shown that anti miR PNA can modulate gene expression and apoptosis in cancer cells.
6) Using properly designed PNAs, in particular chiral PNAs, several probes have been developed for the detection of DNA of biomedical interest or for food analysis. Chiral PNAs turned out to be very useful for the detection of point mutations. In this field, several techniques have been employed, namely surface plasmon resonance (SPR), SPR imaging, microarray technologies, photonic crystal fibers, chromatographic and fluorimetric methods. In particular, the group has been pioneering the field of photonic crystal fibers (PCF) as genosensors, providing the chemistries and detection strategies necessary for this high-tech application. Detection of unamplified genomic DNA have been achieved using SPR imaging and PCF.
7) PNA conjugated to nanozeolites have been produced in collaboration with Prof. L. de Cola (Univ. of Strasbourg), showing for the first time their ability to deliver both PNA and drugs within cells, with increased apoptosis in drug resistant glioma cells. Metal organic frameworks (MOF) have also been modified with model PNA in a collaborative work with J. Huskens (Univ. of Twente, NL).
Completion accademic year: 2019/2020
Completion accademic year: 2018/2019
Completion accademic year: 2017/2018
Completion accademic year: 2016/2017
Completion accademic year: 2015/2016
Completion accademic year: 2014/2015
- First cycle degree (DM 270) BIOTECHNOLOGY A.Y. 2019/2020
- First cycle degree (DM 270) BIOTECHNOLOGY A.Y. 2018/2019
- First cycle degree (DM 270) Biotechnology A.Y. 2017/2018
- First cycle degree (DM 270) Biotechnology A.Y. 2016/2017
- First cycle degree (DM 270) Biotechnology A.Y. 2015/2016
- First cycle degree (DM 270) Biotechnology A.Y. 2014/2015
- First cycle degree (DM 270) Biotechnology A.Y. 2013/2014
Year: 2019Author/s: Finotti, Alessia, Gasparello, Jessica, Fabbri, Enrica, Tamanini, Anna, Corradini, Roberto, Dechecchi, Maria Cristina, Cabrini, Giulio, Gambari, Roberto
64 Cu and fluorescein labeled anti-miRNA peptide nucleic acids for the detection of miRNA expression in living cellsYear: 2019Author/s: Croci, Stefania, Manicardi, Alex, Rubagotti, Sara, Bonacini, Martina, Iori, Michele, Capponi, Pier Cesare, Cicoria, Gianfranco, PARMEGGIANI, MARIA, Salvarani, Carlo, Versari, Annibale, Corradini, Roberto, Asti, Mattia
Year: 2019Author/s: Giovanardi F., Cucinotta A., Rozzi A., Corradini R., Benabid F., Rosa L., Vincetti L.
Targeting miR‑155‑5p and miR‑221‑3p by peptide nucleic acids induces caspase‑3 activation and apoptosis in temozolomide‑resistant T98G glioma cellsYear: 2019Author/s: Milani R., Brognara E., Fabbri E., Manicardi A., Corradini R., Finotti A., Gasparello J., Borgatti M., Cosenza L. C., Lampronti I., Dechecchi M. C., Cabrini G., Gambari R.
Year: 2019Author/s: Gasparello, Jessica, Manicardi, Alex, Casnati, Alessandro, Corradini, Roberto, Gambari, Roberto, Finotti, Alessia, Sansone, Francesco